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INDIAN COUNCIL OF FORESTRY RESEARCH AND EDUCATION has floated a tender for Dna Extraction Kit for Molecular Biology, Qty: 6 50preps, (BOQ Item #8). The project location is Bangalore, Karnataka, India. The reference number is GEM/2024/B/4638284 and it is closing on 26 Feb 2024. Suppliers can request Register free of cost to get the complete Tender details and download the document.
Procurement Summary
State : Karnataka
Summary : Dna Extraction Kit for Molecular Biology, Qty: 6 50preps, (BOQ Item #8)
Deadline : 26 Feb 2024
Other Information
Notice Type : Tender
TOT Ref.No.: 96986521
Document Ref. No. : GEM/2024/B/4638284
Competition : NCB
Financier : Self Financed
Purchaser Ownership : Public
Document Fees : Refer Document
Tender Value : Refer Document
EMD : Refer Document
Purchaser's Detail
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Login to see detailsTender Details
BOQ Item Description: For Molecular Biology, this kit simplifies isolation of DNA from fresh plant material with spin column procedure. The procedure is optimized for a maximum of 200 mg of wet weight of the starting material. The sample (fresh) is cut and ground in liquid nitrogen along with Super Plant Extraction Buffer. Super Plant Extraction Buffer contains CTAB Cetyltrimethylammonium bromide, a detergent used to break open plant cells and solubilize the contents. Chlorophyll and some denatured proteins are removed from green plant tissue in an organic chloroform isoamyl alcohol step, and the organic phase is separated by centrifugation. Since the extract contains DNA and RNA, RNA can be removed by the addition of RNase A. The flow through fraction is then mixed with a solution that enhances the binding of DNA to the column. The solution is then passed through HiElute Miniprep Spin Column Capped that is followed by washing steps to remove trace contaminants. High quality DNA is eluted in the Elution Buffer (ET) provided in the kit.
BOQ Title: Purchase of Chemicals
Item Title: Dna Extraction Kit for Molecular Biology
Item Quantity: 6
Unit of Measure: 50preps
Delivery Period (In number of days): 15
Start Date: 15-02-2024 12:19 PM
End Date: 26-02-2024 12:00 PM
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