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Detection of Global Proteins and Mrna in Postnatal Heart Targeted Adcy8 Overexpression... Tender

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HEALTH AND HUMAN SERVICES, DEPARTMENT OF has floated a tender for Detection of Global Proteins and Mrna in Postnatal Heart Targeted Adcy8 Overexpression Mouse Strain. The project location is USA and the tender is closing on 06 Aug 2024. The tender notice number is 75N95024R00109, while the TOT Ref Number is 104656985. Bidders can have further information about the Tender and can request the complete Tender document by Registering on the site.

Procurement Summary

Country : USA

Summary : Detection of Global Proteins and Mrna in Postnatal Heart Targeted Adcy8 Overexpression Mouse Strain

Deadline : 06 Aug 2024

Other Information

Notice Type : Tender

TOT Ref.No.: 104656985

Document Ref. No. : 75N95024R00109

Competition : ICB

Financier : Self Financed

Purchaser Ownership : Public

Tender Value : Refer Document

Purchaser's Detail

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Tender Details

Description

This is a Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding the availability and capability of all qualified sources to perform a potential requirement.

This notice is issued to help determine the availability of qualified companies technically capable of meeting the Government requirement to determine the method of acquisition. It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract. Responses will not be considered as proposals or quotes. No award will be made as a result of this notice. The Government will NOT be responsible for any costs incurred by the respondents to this notice. This notice is strictly for research and information purposes only. Small businesses are encouraged to respond.

Background: The overexpression of adenylyl cyclase in the mouse heart produces a strong beating heart with the upregulation of many pathways. Global analysis of proteins that emerge are central for the heart survival and regulation of the cell cycle and mitosis check points.

NIA plans to scan global proteins and mRNA by detecting its binding partners in the AdCy8 overexpression model. This analysis will be done through the use of a special method called immunoprecipitation (IP) followed by mass spectrometry analysis (MS), mRNA seq and comparison of the results with the wild type mouse model.

Purpose and Objectives: The purpose of this project is to study postnatal protein expression and mRNA signals in AC8 models in order to illucidate their pathways and their mechanisms in heart cell survival, cell renewal and cell mitosis and its regulation.

Project requirements: Method development and testing of Immuno precipitation (IP) (24 samples), MS analysis (16 samples) and mRNA seq (24 samples). The requirement for IP-LC/MS is to provide biochemical identification, relative quant...
Active Contract Opportunity Notice ID 75N95024R00109 Related Notice Department/Ind. Agency HEALTH AND HUMAN SERVICES, DEPARTMENT OF Sub-tier NATIONAL INSTITUTES OF HEALTH Office NATIONAL INSTITUTES OF HEALTH NIA
General Information
Contract Opportunity Type: Sources Sought (Original)
All Dates/Times are: (UTC-04:00) EASTERN STANDARD TIME, NEW YORK, USA
Original Published Date: Jul 23, 2024 04:03 pm EDT
Original Response Date: Aug 06, 2024 04:00 pm EDT
Inactive Policy: 15 days after response date
Original Inactive Date: Aug 21, 2024
Initiative: None

Classification
Original Set Aside:
Product Service Code: Q301 - REFERENCE LABORATORY TESTING
NAICS Code: 541380 - Testing Laboratories and Services

Place of Performance: Baltimore, MD 21224 USA