Procurement Summary
Country : USA
Summary : Illumina Genome Sequencing
Deadline : 03 Jul 2024
Other Information
Notice Type : Tender
TOT Ref.No.: 103350285
Document Ref. No. : 1132287-SS
Competition : ICB
Financier : Self Financed
Purchaser Ownership : Public
Tender Value : Refer Document
Purchaser's Detail
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Login to see detailsTender Details
Description
Sources Sought #1132287-SS
This is a Sources Sought notice Only. This is NOT a solicitation for proposals, proposal abstracts, or quotations. This Sources Sought is issued for information and planning purposes only as part of USDA ARS market research and does not itself constitute an RFQ.
Background
The USDA ARS NCCCWA requires 30x coverage of whole genome sequencing of 96 DNA samples of rainbow trout (Genome size
2.4Gb).
Contractor shall provide all services, supplies, equipment and facilities necessary to provide sequencing of 96 genomic DNA samples in accordance with the required performance elements and benchmarks.
Schedule of Line Items:
Line Item 01 96 Samples
All 96 DNA samples will be provided within 60 days after the contract is awarded.
Required performance elements and benchmarks:
Amount of DNA provided by the government per sample will be a minimum of 2, 000 ng of rainbow trout genomic DNA. DNA sample quantification and QC will include Qubit and Nano Drop;
Quality check of DNA samples by vendor using Qubit or similar instruments;
Standard Illumina shotgun genomic DNA PCR-free library preparation performed by the vendor (Mean insert size shall be about 500bp);
Platform and configuration: Illumina technology, PE 150 cycles;
Read Length: 2 x 150 nucleotides (paired-end);
Data Output: A minimum of 72 Gbases output DNA sequence per sample from 480M paired-end reads (240M reads in each direction). For each read, greater than 85% of bases shall have QC≥30. The output data files are in standard fastq format;
Vendor must have in place a series of quality control (QC) steps during libraries preparation and genomic DNA sequencing.
Deliverables:
Completion and return of all deliverables are required within 30 days from receipt of all 96 DNA samples. The order number shall be included on all documentation and shipments.
Includes the DNA sequencing data for...
Active Contract Opportunity Notice ID 1132287-SS Related Notice Department/Ind. Agency AGRICULTURE, DEPARTMENT OF Sub-tier AGRICULTURAL RESEARCH SERVICE Office USDA ARS NEA AAO ACQ/PER PROP
General Information
Contract Opportunity Type: Sources Sought (Original)
All Dates/Times are: (UTC-04:00) EASTERN STANDARD TIME, NEW YORK, USA
Original Published Date: Jun 27, 2024 05:16 pm EDT
Original Response Date: Jul 03, 2024 02:00 pm EDT
Inactive Policy: 15 days after response date
Original Inactive Date: Jul 18, 2024
Initiative: None
Classification
Original Set Aside:
Product Service Code: B529 - SPECIAL STUDIES/ANALYSIS- SCIENTIFIC DATA
NAICS Code: 541380 - Testing Laboratories and Services
Place of Performance: Kearneysville, WV 25430 USA
Documents
Tender Notice