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Illumina Genome Sequencing Tender, USA - 103350285

AGRICULTURE, DEPARTMENT OF has floated a tender for Illumina Genome Sequencing. The project location is USA and the tender is closing on 03 Jul 2024. The tender notice number is 1132287-SS, while the TOT Ref Number is 103350285. Bidders can have further information about the Tender and can request the complete Tender document by Registering on the site.

Procurement Summary

Country : USA

Summary : Illumina Genome Sequencing

Deadline : 03 Jul 2024

Other Information

Notice Type : Tender

TOT Ref.No.: 103350285

Document Ref. No. : 1132287-SS

Competition : ICB

Financier : Self Financed

Purchaser Ownership : Public

Tender Value : Refer Document

Purchaser's Detail

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Tender Details

Description

Sources Sought #1132287-SS

This is a Sources Sought notice Only. This is NOT a solicitation for proposals, proposal abstracts, or quotations. This Sources Sought is issued for information and planning purposes only as part of USDA ARS market research and does not itself constitute an RFQ.

Background

The USDA ARS NCCCWA requires 30X coverage of whole genome sequencing of 96 DNA samples of rainbow trout (Genome size

2.4Gb).

Contractor shall provide all services, supplies, equipment and facilities necessary to provide sequencing of 96 genomic DNA samples in accordance with the required performance elements and benchmarks.

Schedule of Line Items:

Line Item 01 96 Samples

All 96 DNA samples will be provided within 60 days after the contract is awarded.

Required performance elements and benchmarks:

Amount of DNA provided by the government per sample will be a minimum of 2, 000 ng of rainbow trout genomic DNA. DNA sample quantification and QC will include Qubit and Nano Drop;

Quality check of DNA samples by vendor using Qubit or similar instruments;

Standard Illumina shotgun genomic DNA PCR-free library preparation performed by the vendor (Mean insert size shall be about 500bp);

Platform and configuration: Illumina technology, PE 150 cycles;

Read Length: 2 x 150 nucleotides (paired-end);

Data Output: A minimum of 72 Gbases output DNA sequence per sample from 480M paired-end reads (240M reads in each direction). For each read, greater than 85% of bases shall have QC≥30. The output data files are in standard fastq format;

Vendor must have in place a series of quality control (QC) steps during libraries preparation and genomic DNA sequencing.

Deliverables:

Completion and return of all deliverables are required within 30 days from receipt of all 96 DNA samples. The order number shall be included on all documentation and shipments.

Includes the DNA sequencing data for...
Active Contract Opportunity Notice ID 1132287-SS Related Notice Department/Ind. Agency AGRICULTURE, DEPARTMENT OF Sub-tier AGRICULTURAL RESEARCH SERVICE Office USDA ARS NEA AAO ACQ/PER PROP
General Information
Contract Opportunity Type: Sources Sought (Original)
All Dates/Times are: (UTC-04:00) EASTERN STANDARD TIME, NEW YORK, USA
Original Published Date: Jun 27, 2024 05:16 pm EDT
Original Response Date: Jul 03, 2024 02:00 pm EDT
Inactive Policy: 15 days after response date
Original Inactive Date: Jul 18, 2024
Initiative: None

Classification
Original Set Aside:
Product Service Code: B529 - SPECIAL STUDIES/ANALYSIS- SCIENTIFIC DATA
NAICS Code: 541380 - Testing Laboratories and Services

Place of Performance: Kearneysville, WV 25430 USA