Services for Probe Design and Rnascope Brand Reagent Kits Tender, USA - 81724167

Procurement Summary

Country : USA

Summary : Services for Probe Design and Rnascope Brand Reagent Kits

Deadline : 22 Apr 2023

Other Information

Notice Type : Tender

TOT Ref.No.: 81724167

Document Ref. No. : NOI-NIAID-2169561

Competition : ICB

Financier : Self Financed

Purchaser Ownership : Public

Tender Value : Refer Document

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Tender Details

Description

This is a Notice of Intent, not a request for proposal. The National Institute of Allergy and Infectious Diseases (NIAID) of the National Institutes of Health (NIH) intends to negotiate on an other than full and open competition basis with a Advanced Cell Diagnostics, Newark, CA

These services and supplies are required for RNA in situ hybridization.Qty: 23 Bioinformatics service fee for probe designs (Multiple) Qty: 23 RNASCOPE brand probes (Multiple)Qty: 1 RNASCOPE brand Multiplex reagent kit #323270

Sole Source Justification: Advanced Cell Diagnostics, Inc. (ACD) is the sole supplier of RNAscope® probes and reagent Kits.

This projects demands RNA in situ hybridization to validate 23 target markers and visualize the individual cellular clusters identified in the single-cell RNA-seq analysis. RNAscope enables as much as 400-fold improvement over conventional RNA in situ hybridization, resulting in high detection sensitivity, thus RNAscope technology is the best choice to accomplish our goals for this project. RNAscope was designed to amplify target-specific signal without also amplifying the background signal, resulting in marked improvement in signal-to-noise ratio. This is accomplished by manufacture`s patented double Z-probe design for in situ hybridization, where two independent probes (a double Z probe pair) are required to hybridize in tandem to the target sequence for signal amplification to occur. Since it is highly unlikely that two independent probes will hybridize to a nonspecific target right next to each other, this design concept ensures highly selective amplification of target-specific signals, therefore improving both sensitivity and specificity. A Preamplifier (PreAmp) molecule hybridizes to each double Z probe pair, and then multiple Amplifier (AMP) molecules hybridize to each Preamp. Finally, multiple HRP-labeled Label Probes hybridized to each AMP. DAB substrate is added for colorimetric detection of target RNA. Fluor...
Active Contract Opportunity
Notice ID : NOI-NIAID-2169561
Related Notice
Department/Ind. Agency : HEALTH AND HUMAN SERVICES, DEPARTMENT OF
Sub-tier : NATIONAL INSTITUTES OF HEALTH
Office : NATIONAL INSTITUTES OF HEALTH NIAID
General Information
Contract Opportunity Type: Presolicitation (Original)
All Dates/Times are: (UTC-04:00) EASTERN STANDARD TIME, NEW YORK, USA
Original Published Date: Apr 15, 2023 12:57 pm EDT
Original Response Date: Apr 22, 2023 04:00 pm EDT
Inactive Policy: 15 days after response date
Original Inactive Date:
Initiative: None

Classification
Original Set Aside:
Product Service Code: 6550 - IN VITRO DIAGNOSTIC SUBSTANCES, REAGENTS, TEST KITS AND SETS
NAICS Code: 325414 - Biological Product (except Diagnostic) Manufacturing

Place of Performance: Hamilton, MT 59840 USA

Documents

 Tender Notice