Sources Sought for Sequencing of Wild Pea, Pisum Fulvum - Pfna27 Reference Genome Tender

Procurement Summary

Country : USA

Summary : Sources Sought for Sequencing of Wild Pea, Pisum Fulvum - Pfna27 Reference Genome

Deadline : 19 Jun 2024

Other Information

Notice Type : Tender

TOT Ref.No.: 102679561

Document Ref. No. : 12905B24SS00040

Competition : ICB

Financier : Self Financed

Purchaser Ownership : Public

Tender Value : Refer Document

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Tender Details

Description

This is a Sources Sought notice Only. This is NOT a solicitation for proposals, proposal abstracts, or quotations. This Sources Sought is issued for information and planning purposes only as part of USDA ARS market research and does not itself constitute an RFQ. This requirement is covered under NAICS code 541714. The purpose of this notice is to obtain information regarding the availability and capability of qualified sources; Small Businesses; HUBZone small business; service-disabled, veteran-owned small business; 8(a) small business; veteran-owned small business; woman-owned small business; or small disadvantaged businesses; Responses to the information requested will assist the Government in determining the appropriate acquisition method, including whether a set-aside is possible.



The Sequence and assembly of high-quality complete reference genome with annotated transcriptome of USDA ARS Accession Number PI 343996 pea Pisum sativum subspecies elatius using the PacBio Revio system high-density 25 million ZMW SMRT cells delivering HiFi reads. Work to include library construction frozen plant tissue using the Dovetail Hi-C Kit. Expected product will include the yield for CCS sequencing of 60-90Gb for the pea DNA per lane and a final yield of 2.5 billion read pairs and 750Gb of raw data per lane. Gene and protein annotation will be included. In addition, RNA prep from frozen plant tissue (8 different plant tissues, see list below), RNASeq library construction and sequencing of up to 40 million read pairs per sample using Stranded HT, Illumina Sequencing on a NovaSeq 6000 PE150. Data for the complete genome annotation will be delivered as GFF, cDNA and protein files for each pea sample (haplotype). Base pair call accuracy of 99.9% (Q30 or greater).



For each genotype, the sequencing costs $189.00 for first 10 Gb and $95.00 for each incremental 10Gb. Therefore, for 40Gb data, the total cost per genotype is $474 [189 + (3*95)]. Total c...
Active Contract Opportunity Notice ID 12905B24SS00040 Related Notice Department/Ind. Agency AGRICULTURE, DEPARTMENT OF Sub-tier AGRICULTURAL RESEARCH SERVICE Office USDA ARS PWA AAO ACQ/PER PROP
General Information
Contract Opportunity Type: Sources Sought (Original)
All Dates/Times are: (UTC-07:00) PACIFIC STANDARD TIME, LOS ANGELES, USA
Original Published Date: Jun 12, 2024 03:22 pm PDT
Original Response Date: Jun 19, 2024 05:00 pm PDT
Inactive Policy: 15 days after response date
Original Inactive Date: Jul 04, 2024
Initiative: None

Classification
Original Set Aside:
Product Service Code: B529 - SPECIAL STUDIES/ANALYSIS- SCIENTIFIC DATA
NAICS Code: 541714 - Research and Development in Biotechnology (except Nanobiotechnology)

Place of Performance: Pullman, WA 99163 USA

Documents

 Tender Notice